The Berro lab is interested in how forces are produced and sensed at the molecular scale in cells. Our research is currently focused on unraveling how the molecular machinery of clathrin-mediated endocytosis generates forces to deform the plasma membrane and conversely how this machinery senses membrane tension and adapts to it. To answer these questions, the lab develops new experimental and computational quantitative methods for cell biology, biochemistry and biophysics.
Today was the last day of our fantastic undergraduate student from @GeorgiaSouthern Katherine Barrs. She was helping us develop a fast assay to measure endo and exocytosis rates in yeast. Thank you Katherine! 👍 pic.twitter.com/52Pth4QtZx
— Berro Lab 🔬📏📉💻 (@BerroLab) August 2, 2019
Julien Berro (@BerroLab; @Yale) is our next 'Cell Scientist to Watch' at @J_Cell_Sci. Read the interview here: https://t.co/OpT9rzWc35
— Manuel Breuer (@JCS_MB) May 2, 2019
The lab latest preprint is online! Our simulations show that crosslinking short actin filaments can produce significant amounts of compressive force https://t.co/Klj3NfB5nZ
— Berro Lab 🔬📏📉💻 (@BerroLab) April 21, 2019
Figures illustrating how crosslinking short actin filaments produces compressive forceshttps://t.co/D6sCb32FKo pic.twitter.com/z4YkYeudM5
— Berro Lab 🔬📏📉💻 (@BerroLab) April 25, 2019
Our new manuscript shows how the force barrier for endocytosis in yeast can be dramatically lowered by changing how the membrane is bound to the cell wall and by partially coating the membranehttps://t.co/IvqHop2OPp
— Berro Lab 🔬📏📉💻 (@BerroLab) February 23, 2019
Check out our new review on the molecular mechanisms of force production in clathrin‐mediated endocytosis! https://t.co/ADGKxo8jK4 pic.twitter.com/on8pRROkhv
— Berro Lab 🔬📏📉💻 (@BerroLab) July 16, 2018
Our book on quantitative methods to study endocytosis is out! https://t.co/M0czqm0i2v@MorganClaypool @WallaceUcsf pic.twitter.com/f0uxiReeng
— Berro Lab 🔬📏📉💻 (@BerroLab) August 15, 2018
New modeling preprint based on some of the very first experiments I ever did in @Lblanchoin lab! https://t.co/fz3SpEulUR
— Berro Lab 🔬📏📉💻 (@BerroLab) June 13, 2018
— Berro Lab 🔬📏📉💻 (@BerroLab) June 13, 2018
The Berro lab has a new doctor in the house. Congrats Mike Lacy! pic.twitter.com/iofCIf0Xx8
— Berro Lab 🔬📏📉💻 (@BerroLab) May 22, 2018
Our paper on the conversion of binding energy into elastic energy via actin filament crosslinking is now published in @PLOSCompBiol: Structural organization and energy storage in crosslinked actin assemblies https://t.co/3SGktFCeB4
— Berro Lab 🔬📏📉💻 (@BerroLab) May 31, 2018
This coffee-stirrer-and-spring model summarizes it all! pic.twitter.com/JHR2YBNMMN
— Berro Lab 🔬📏📉💻 (@BerroLab) May 31, 2018
Our simulations uncovered new mechanisms for the conversion of binding energy into elastic energy, and subsequently into torque, via dynamic actin filament crosslinking.
https://www.biorxiv.org/content/early/2017/12/20/237412
Mike’s single molecule study of the eisosome has been published in MBoC.
http://www.molbiolcell.org/content/28/17/2251.full
A few plasmids that were created in the Berro lab and can be useful for the S. pombe community are now available on Addgene. These include plasmids to perform CRISPR/Cas9 edition using fluoride selection (Fernandez and Berro, 2016), and plasmids to C-terminally tag proteins with SNAP, CLIP, Halo, mEOS3.2, PAmKate, PATagRFP or PAmCherry (unpublished).