Publications

2024

Maria Tsemperouli^†, Sudheer Kumar Cheppali^†, Felix Rivera Molina, David Chetrit, Ane Landajuela, Derek Toomre, and Erdem Karatekin*, “Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1″ (^†equal contribution), bioRxiv 2024.09.12.612660; doi: https://doi.org/10.1101/2024.09.12.612660
Jessica J. Mohsen, Michael G. Mohsen, Kevin Jiang, Ane Landajuela, Laura Quinto, Farren J. Isaacs, Erdem Karatekin, Sarah A. Slavoff*, “Cellular function of the GndA small open reading frame-encoded polypeptide during heat shock”, bioRx

iv 2024.06.29.601336;doi: https://doi.org/10.1101/2024.06.29.601336
Ane Landajuela*, Martha Braun, Christopher D.A. Rodrigues, and Erdem Karatekin*, “Detection of membrane fission in single Bacillus subtilis cells during endospore formation with high temporal resolution”, STAR Protocols,2024 Mar 18;5(2):102965. doi: 10.1016/j.xpro.2024.102965.
Qi Yan, Carolina Gomis Perez, and Erdem Karatekin*, “Membrane tension gradients, cell membrane flows and cellular processes”, Physiology (Am. Physiological Soc.), https://journals.physiology.org/doi/abs/10.1152/physiol.00007.2024.

2023

Zachary McDargh, Shuyuan Wang, Harvey F. Chin, Sathish Thiyagarajan, Erdem Karatekin, Thomas D. Pollard, Ben O’Shaughnessy*, “Myosins generate contractile force and maintain organization in the cytokinetic contractile ring”, bioRxiv 2021.05.02.442363; doi: https://doi.org/10.1101/2021.05.02.442363.
D. Xu, W. Jiang, L. Wu, R.G. Gaudet, E.S. Park, M. Su, S.K. Cheppali, N.R. Cheemarla, P. Kumar, P.D. Uchil, J.R. Grover, E.F. Foxman, C.M. Brown, P.J. Stansfeld, J. Bewersdorf , W. Mothes, E. Karatekin, C.B. Wilen, J.D. MacMicking*. “PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection.” Nature, (2023), 619:819–827. https://doi.org/10.1038/s41586-023-06322-y PMID: 37438530

2022

Ane Landajuela^†*, Martha Braun^†*, Alejandro Martínez-Calvo, Christopher D. A. Rodrigues, Carolina Gomis Perez, Thierry Doan, David Z. Rudner, Ned S. Wingreen, and Erdem Karatekin*, (^†equal contribution; *co-corresponding authors), “Membrane fission during bacterial spore development requires cellular inflation driven by DNA translocation”, Curr Biol. (2022); 32:4186-4200.e8. doi: 10.1016/j.cub.2022.08.014. PMID: 36041438
Zhenyong Wu, Lu Ma, Nicholas A. Courtney, Jie Zhu, Ane Landajuela, Yongli Zhang*, Edwin R. Chapman*, and Erdem Karatekin*, “Polybasic patches in both C2 domains of Synaptotagmin-1 are required for evoked neurotransmitter release“, J Neurosci. (2022); 42:5816-5829. doi: 10.1523/JNEUROSCI.1385-21.2022. PMID: 35701163
J. Ge, X. Bian, L. Ma, Y. Cai, Y. Li, J. Yang, Y. Saheki, E. Karatekin, P. De Camilli, Y. Zhang*, “Stepwise membrane binding of the C2 domains of the Extended Synaptotagmins revealed by optical tweezers”. Nat. Chem. Biol. (2022), 18:313–320.
Carolina Gomis Perez^†, Natasha R. Dudzinski^†, Mason Rouches, Ane Landajuela, Benjamin Machta, David Zenisek, Erdem Karatekin* (^†equal contribution), “Rapid propagation of membrane tension at a presynaptic terminal”, Sci. Advances. https://www.science.org/doi/10.1126/sciadv.abl4411

2021

Joerg Nikolaus, Kasey Hancock, Maria Tsemperouli, David Baddeley*, and Erdem Karatekin*, “Optimal Detection of fusion pore dynamics using polarized total internal reflection fluorescence microscopy”, Front. Mol. Biosci. (2021), https://doi.org/10.3389/fmolb.2021.740408.
Ryan G. Gaudet, Shiwei Zhu, Anushka Halder, Bae-Hoon Kim, Clinton J. Bradfield, Shuai Huang, Dijin Xu, Agnieszka Mamiñska, Thanh Ngoc Nguyen, Michael Lazarou, Erdem Karatekin, Kallol Gupta, and John D. MacMicking*, “A human apolipoprotein L with detergent-like activity kills intracellular pathogens”, Science (2021), 373 (6552): eabf8113, DOI: 10.1126/science.abf8113. See Perspective: Science (2021) v. 373 (6552), pp. 276-277, DOI: 10.1126/science.abj5637.
Zhenyong Wu, Nadiv Dharan, Sathish Thiyagarajan, Ben O’Shaughnessy*, Erdem Karatekin*, “The neuronal calcium sensor Synaptotagmin-1 and SNARE proteins cooperate to dilate fusion pores”, eLife 2021;10:e68215. DOI: https://doi.org/10.7554/eLife.68215.
Ane Landajuela^†*, Martha Braun^†, Christopher D. A. Rodrigues, Thierry Doan, Florian Horenkamp, Anna Andronicos, Vladimir Shteyn, Nathan D. Williams, Chenxiang Lin, David Z. Rudner, Erdem Karatekin* (^†equal contribution; *co-corresponding authors), “The bacterial membrane fission protein FisB requires homo-oligomerization and lipid-binding to catalyze membrane scission“, PLoS Biol. (2021) 19(6): e3001314. https://doi.org/10.1371/journal.pbio.3001314.
Yang Yang, Zhenyong Wu, Laurie Wang, Kaifeng Zhou, Kai Xia, Qiancheng Xiong, Longfei Liu, Zhao Zhang, Edwin R. Chapman, Yong Xiong, Thomas J. Melia, Erdem Karatekin, Hongzhou Gu* & Chenxiang Lin*, “Sorting sub-150-nm liposomes of distinct sizes by DNA-brick-assisted centrifugation”, Nat. Chem. 13:335-342 (2021). https://doi.org/10.1038/s41557-021-00667-5

2020

Nathan D. Williams, Ane Landajuela, Ravi Kiran Kasula, Wenjiao Zhou, John T. Powell, Zhiqun Xi, Farren J. Isaacs, Julien Berro, Derek Toomre, Erdem Karatekin, Chenxiang Lin*, “DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells”, Nano Letters 2020.
Catherine L. Deatherage, Joerg Nikolaus, Erdem Karatekin*, Christopher G. Burd*, “Retromer forms low order oligomers on supported lipid bilayers.”J. Biol. Chem., 2020; 295:12305–12316. doi: 10.1074/jbc.RA120.013672.

2019

Chunguang Ren, Qianying Yuan, Martha Braun, Xia Zhang, Bjorn Petri, Jiasheng Zhang, Dongjoo Kim, Julia Guez-Haddad, Wenzhi Xue, Weijun Pan, Rong Fan, Paul Kubes, Zhaoxia Sun, Yarden Opatowsky, Franck Polleux, Erdem Karatekin*, Wenwen Tang*, and Dianqing Wu*, “Leukocyte Cytoskeleton Polarization Is Initiated by Plasma Membrane Curvature from Cell Attachment”, Dev. Cell, 2019; 49:206–219. https://doi.org/10.1016/j.devcel.2019.02.023
Natasha R. Dudzinski, Zhenyong Wu*, Erdem Karatekin*, “A Nanodisc-Cell Fusion Assay with Single-Pore Sensitivity and Sub-millisecond Time Resolution”, Methods Mol Biol. 2019;1860:263-275. doi: 10.1007/978-1-4939-8760-3_17.

2018

Erdem Karatekin and James E. Rothman, FEBS Letters Special Issue on Exocytosis and Endocytosis. Editorial. FEBS Lett. 2018, 592:3477-3479. doi: 10.1002/1873-3468.13274.
Erdem Karatekin*, “Toward a unified picture of the exocytotic fusion pore”, FEBS Lett. 2018; 592:3563-3585. doi: 10.1002/1873-3468.13270. Epub 2018 Oct 26.

2017

Lu Ma, Yiying Cai, Yanghui Li, Junyi Jiao, Zhenyong Wu, Ben O’Shaughnessy, Pietro De Camilli*, Erdem Karatekin*, Yongli Zhang*, “Single-molecule force spectroscopy of protein-membrane interactions.”, eLife 2017;6:e30493 doi: 10.7554/eLife.30493
Z. Wu, S. Thiyagarajan, B. O’Shaughnessy and E. Karatekin*, “Regulation of exocytotic fusion pores by SNARE protein transmembrane domains”, Front. Mol. Neurosci. 2017 doi: 10.3389/fnmol.2017.00315
H. Mostafavi, S. Thiyagarajan, B. S. Stratton, E. Karatekin, J. M. Warner,
J. E. Rothman*, and B. O’Shaughnessy*, “Entropic forces drive self-organization and membrane fusion by SNARE proteins“, PNAS 2017 doi/10.1073/pnas.1611506114
Z. Wu, O. Bello, S. Thiyagarajan, S. M. Auclair, W. Vennekate, S. S. Krishnakumar, B. O’Shaughnessy and E. Karatekin*, “Dilation of fusion pores by crowding of SNARE proteins”, eLife 2017;10.7554/eLife.22964.

2016

Z. Wu, S. M. Auclair, O. Bello, W. Vennekate, N. R. Dudzinski, S. S. Krishnakumar, and E. Karatekin*, “Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains”, Scientific Reports, 6, 27287, 2016.
W. Xu, B. Nathwani, C. Lin, J. Wang, E. Karatekin, F. Pincet, W. Shih*, and J. E. Rothman*,“A programmable DNA origami platform to organize SNAREs for membrane fusion”, J. Amer. Chem. Soc. 138:4439-4447, 2016.
J. Nikolaus*, E. Karatekin*, “SNARE-mediated fusion of single proteoliposomes with tethered supported bilayers in a microfluidic flow cell monitored by polarized TIRF microscopy”, J. Vis. Exp. (114), e54349, doi:10.3791/54349 (2016).
B. S. Stratton, J. M. Warner, Z. Wu, J. Nikolaus, G. Wei, E. Wagnon, D. Baddeley, E. Karatekin*, and B. O’Shaughnessy*, “Cholesterol increases the openness of SNARE-mediated flickering fusion pores“, Biophys. J. 110:1538-1550, 2016.

2015

C. Laplante, J. Berro, E. Karatekin, A. Hernandez-Leyva, R. Lee, and T. D. Pollard*, “Three Myosins Contribute Uniquely to the Assembly and Constriction of the Fission Yeast Cytokinetic Contractile Ring“ Current Biology, 25, 1955-1965, 2015.

2014

M. R. Stachowiak, C. Laplante, H. F. Chin, B. Guirao, E. Karatekin, T. D. Pollard, and B. O’Shaughnessy*, “Mechanism of Cytokinetic Contractile Ring Constriction in Fission Yeast”, Dev. Cell, 29, 547–561, 2014.

2013

T. Doan, J. Coleman, K. A. Marquis, B.M. Burton, E. Karatekin*, and D. Z. Rudner*, “FisB mediates the final membrane fission event during sporulation in Bacillus Subtilis”, Genes Dev., 27, 322-334, 2013.

2012

E. Karatekin*and J.E. Rothman, “Fusion of single proteoliposomes with planar, cushioned bilayers in microfluidic flow cells”, Nature Protocols, 7:903-920, 2012.

2011

Smith MB, Karatekin E, Gohlke A, Mizuno H, Watanabe N, and Vavylonis D. “Interactive, computer-assisted tracking of speckle trajectories in fluorescence microscopy: application to actin polymerization and membrane fusion”, Biophys. J. 101:1794-1804, 2011.
Meunier A, Jouannot O, Fulcrand R, Fanget I, Bretou M, Karatekin E, Arbault S, Guille M, Darchen F, Lemaître F, and Amatore C., “Coupling amperometry and total internal reflection fluorescence microscopy at ITO surfaces for monitoring exocytosis of single vesicles”, Angew. Chem. Int. Ed., 50:5081-5084, 2011.

2010

Karatekin E, Di Giovanni J, Iborra C, Coleman J, O’Shaughnessy B, Seagar M, and Rothman JE., “A fast, single-vesicle fusion assay mimics physiological SNARE requirements”, Proc. Natl. Acad. Sci. USA. 107:3517–3521, 2010.

2009

Warner JM, Karatekin E, and O’Shaughnessy B. “Model of SNARE-mediated membrane adhesion kinetics”, PLoS One 4(8):e6375, 2009.

2008

Karatekin E, Tran S, Huet S, Fanget I, Cribier S, and Henry JP., “A 20 nm step toward the cell membrane preceding exocytosis may correspond to docking of tethered granules”, Biophys. J. 94:2891-2905, 2008.

pre-2008

Tran VS, Huet S, Fanget I, Cribier S, Henry JP, and Karatekin E., “Characterization of sequential exocytosis in a human neuroendocrine cell line using evanescent wave microscopy and `virtual trajectory’ analysis”, Eur. Biophys. J. 37:55-69, 2007.
S. Huet, E. Karatekin*, V. S. Tran, I. Fanget, S. Cribier and J.-P. Henry*, “Analysis of transient behavior in complex trajectories: application to secretory vesicle dynamics.” Biophys. J. 91, 3542-3559 (2006).
N. Borghi, F. Brochard-Wyart, E. Karatekin, and I. Derényi, “Giant vesicles: Transient pores and tube extraction”. Proc. Int. School of Physics “Enrico Fermi”. Course CLV, F. Mallamace and H. E. Stanley (Eds.); IOS Press, Amsterdam (2004).
L. Zecca*, A. Stroppolo, A. Gatti, D. Tampellini, M. Toscani, M. Gallorini, G. Giaveri, P. Arosio, P. Santambrogio, R. G. Fariello, E. Karatekin, M. H. Kleinman, N. Turro, O. Hornykiewicz, and F. A. Zucca, “The role of iron and copper molecules in the neuronal vulnerability of locus coeruleus and substantia nigra during aging”. Proc. Natl. Acad. Sci. USA, 101, 9843-9848 (2004).
S. Tran, A.-M. Marion-Audibert, E. Karatekin, S. Huet, S. Cribier, K. Guillaumie, C. Chapuis, C. Desnos, F. Darchen, and J.-P. Henry*, “Serotonin secretion by human carcinoid BON cells”, Ann. N.Y. Acad. Sci., 1014, 1–10 (2004).
P.-H. Puech, N. Borghi, E. Karatekin, and F. Brochard-Wyart*, “Line Thermodynamics: Adsorption at a Membrane Edge”, Phys. Rev. Lett., 90, 128304 (2003). Highlighted as a Search and Discovery article: “Experiments Investigate the Behavior of Pores in Artificial Vesicles: Twenty-first century experimental techniques combine with 19th century theory to explore how cell membranes behave when breached.” Physics Today, 56 (5):22-24, May 2003.
E. Karatekin, O. Sandre, H. Guitouni, N. Borghi, P.-H. Puech, and F. Brochard-Wyart*, “Cascades of Transient Pores in Giant Vesicles: Line Tension and Transport”, Biophys. J., 84, 1734-1749 (2003).
E. Karatekin, O. Sandre, and F. Brochard-Wyart*, “Transient Pores in Vesicles”, Polym. Int., 52, 486-493 (2003).
E. Karatekin, B. O’Shaughnessy*, and N. J. Turro, “The Photocopy Method: Measuring Living Chain Distributions in Radical Polymerization”, Macromol. Symp., 182, 81-101 (2002).
E. Karatekin, M. S. Landis, G. Lem, B. O’Shaughnessy*, and N. J. Turro, “Photocopying Living Chains. 2. Time-Dependent Measurements”, Macromolecules, 34, 8202-8215 (2001).
E. Karatekin, M. S. Landis, G. Lem, B. O’Shaughnessy*, and N. J. Turro, “Photocopying Living Chains. 1. Steady-State”, Macromolecules, 34, 8187-8201 (2001).
N. J. Turro*, M. H. Kleinman, and E. Karatekin, “Electron Spin Polarization and Time-Resolved Electron Paramagnetic Resonance: Applications to the Paradigms of Molecular and Supramolecular Photochemistry”, Angew. Chem. Int. Ed., 39, 4436-4461 (2000).
D. Sulzer*, J. Bogulavsky, K. E. Larsen, G. Behr, E. Karatekin, M. H. Kleinman, N. Turro, D. Krantz, R. H. Edwards, L. A. Greene, L. Zecca, “Neuromelanin Biosynthesis is Driven by Excess Cytosolic Catecholamines not Accumulated by Synaptic Vesicles”. Proc. Natl. Acad. Sci. USA, 97, 11869-11874 (2000).
E. Karatekin, B. O’Shaughnessy, and N. J. Turro*, “Time Resolved EPR: A Novel Method for Studying Living Chains”, Macromolecules, 31, 7992-7995 (1998).
E. Karatekin, B. O’Shaughnessy*, and N. J. Turro, “Kinetic Isolation of Macroradicals”, Macromolecules, 31, 4655-4658 (1998).
E. Karatekin, B. O’Shaughnessy*, and N. J. Turro, “Kinetic Isolation of Persistent Radicals and Application to Polymer-Polymer Reactions”, J. Chem. Phys., 108, 9577-9585 (1998).